Purification and Characterization of an Endo-cellulase from Acremonium cellulolyticus.
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چکیده
منابع مشابه
Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass
BACKGROUND Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide clues for maximizing the hydrolysis of target biomass. Talaromyces cellulolyticus is a promising fungus for cellulase production and efficient biomass hydrolysis. Several cellulo...
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Cellulase production was investigated in pH-controlled cultures of Acremonium cellulolyticus. The response to culture pH was investigated for three cellulolytic enzymes, carbomethyl cellulase (CMCase), avicelase, and beta-glucosidase. Avicelase and beta-glucosidase showed similar profiles, with maximum activity in cultures at pH 5.5-6. The CMCase activity was highest in a pH 4 culture. At an ac...
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13 Cellulase production in cultures of Acremonium cellulolyticus was significantly 14 improved by using waste milk pack (MP) that had been pretreated with cellulase. When 15 MP cellulose pretreated with cellulase (3 FPU/g MP) for 12 h was used as the sole 16 carbon source for A. cellulolyticus culture in a 3-L fermentor, the cellulase activity was 17 16 FPU/ml. This was 25-fold higher (0.67 FPU...
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Carboxymethyl cellulase (CMCase) is the key enzyme used by bacteria to decompose the plant root hairwall during symbiosis. In this study, CMCase activities were routinely assayed using carboxymethyl cellulose (CMC) as a substrate. Coupled with the catalytic reaction of BCA (2,2’-bicinchoninic acid) solution, the color developed at 570 nm was measured to determine the enzyme activity. Sinorhizob...
متن کاملCrystal Structure of Talaromyces cellulolyticus (Formerly Known as Acremonium cellulolyticus) GH Family 11 Xylanase
Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus) is one of the mesophilic fungi that can produce high levels of cellulose-related enzymes and are expected to be used for the degradation of polysaccharide biomass. In silico analysis of the genome sequence of T. cellulolyticus detected seven open reading frames (ORFs) showing homology to xylanases from glycoside hydrolase ...
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ژورنال
عنوان ژورنال: Journal of Applied Glycoscience
سال: 2000
ISSN: 1344-7882,1880-7291
DOI: 10.5458/jag.47.177